Table 2.

Laccase activity (µmol ABTS^•+ kg⁻¹ min⁻¹ ± SD), and the concentrations of protein, carbohydrate, and total phenol (mg kg⁻¹) in the four consecutive washing fluids and inside the washed and milled basidiospores of S. rugoso-annulata and K. mutabilis (n = 2–4).

Species	Extract	Laccase	Protein	Carbohydrate	Total Phenol c
Stropharia	1. Wash	32.8 ± 3.90	21 ± 99	7270 ± 33 a	18.5 ± 2.1
	2. Wash	7.38 ± 2.56	ND	ND	18.8 ± 3.6
	3. Wash	1.34 ± 1.34	ND	ND	13.3 ± 1.8
	4. Wash	0.28 ± 0.20	ND	ND	10.9 ± 0.1
	Total 1–4	41.7 ± 4.9	21 ± 99	7270 ± 33 a	61.5 ± 4.6
	Inside concentr.	688 ± 84	12,600 ± 405	160,000 ± 2670 a 96,600 ± 1200 b	452 ± 53
Kuehneromyces	1. Wash	0.15 ± 0.15	168 ± 121	9730 a	68.3 ± 6.3
	2. Wash	ND	ND	ND	73.6 ± 6.9
	3. Wash	ND	ND	ND	36.9 ± 2.5
	4. Wash	ND	ND	ND	40.2 ± 0.5
	Total 1–4	0.15 ± 0.15	168 ± 121	9730 a	219 ± 9.7
	Inside concentr.	0.30 ± 0.03	924 ± 49	15,130 ± 490 a 6750 ± 56 b	767 ± 27

^a Expressed in sucrose equivalents; ^b in xylose equivalents; ^c in pyrogallol equivalents. ND, not detected.