Table 6.

Activity ranges of unwashed and aseptically germinating K. mutabilis basidiospores in 6 mL of high-purity solutions of glucose, guaiacol, and ABTS determined at 11 to 12 d (n = 3–4).

Spore Incubation Medium Amended with (L−1)	Relative Mycelial Quantity	Plateau Concentr. of Product µM	Enzymatic ABTS Oxidation in µM Per53 h b Per Min a		Protein Released g kg−1 Spores	Rest Glucose g L−1	Mean Final pH of Spore Medium
Spore load 3–4 mg per flask (0.5–0.67 g L−1)
Glucose 5 g	1		3.4–35		0.6–1.7 2	2.5–3 2	2.6
Guaiacol 133 mg	1.3	64–133 1		0.7–1.3 1	4.4–5.3 1	0 1	2.8
Glucose/guaiacol	0.8–1.7	16–25 2	6.6	0.14 2	1.8–3.0 2	3.5–3.8 2	2.7
Spore load < 0.3 mg per flask (<0.05 g L−1)
ABTS 224 mg	<0.1	1.9–47	ND	ND	ND	0
Glucose/ABTS	<0.1	2.3–67	ND	ND	ND	4.5–5
Glucose 5 g	2 c			2.3–6.1 c			3.8 c

^a Enzyme activities (µM min⁻¹) are re-calculated for 100% of spore incubation medium in the reaction mixture. ^b Values obtained with 0.33 mL of spore incubation medium in 1 mL of reaction mixture. ^c Mycelium with aerial hyphae on the incubation medium. Data ranges with different superscript numbers in the guaiacol treatments differ significantly at p ≤ 0.05. Detection limits, 0.3–0.5 µM for guaicol and 56–83 nM for ABTS transformation products. ND, not determined. Blanks, no corresponding values available.