Figure 3.

Electrophysiology-based bioassay with a two-electrode voltage clamp technique, as measured in Xenopus laevis oocytes. Shown is a validation trace experiment where a G-protein coupled receptor (GPCR) is coupled to an effector channel, an inward rectifier potassium channel via a G_i/o cascade. Currents were induced by exchanging a control saline low potassium solution (ND96 buffer = blue) with a measuring solution with elevated potassium (HK solution = grey). The trace reveals the agonistic activity of a bioactive component originating from a caterpillar (pink). A synthetic agonist was used as control.