Table 1.
Isolate ID | Age | Gender | Onset of Symptoms—dd/mm/yyyy | Collection Date—dd/mm/yyyy | Sample | qRT-PCR Ct a | Genbank Accession Code | Total No. Reads b | No. Mapped Reads (%) b | Depth of Coverage b | Genome Length (nt) b |
---|---|---|---|---|---|---|---|---|---|---|---|
H307 MAYV | 21 | F | 14/03/2015 | 16/03/2015 | C6/36 passage 1 | 6.12 | MH513597 | 2,813,153 | 2,376,618 (84.48%) | 15,453.99 | 11,147 |
H542 CHIKV | 20 | F | 04/02/2018 | 07/02/2018 | Serum | 23.19 | MT349960 | 1,123,424 | 1,083,817 (96.47%) | 7133.15 | 11,492 |
Depth of coverage = number of mapped reads × 75 (read length)/reference genome length (11,534 for MAYV and 11,812 for CHIKV). a Ct, cycle threshold; qRT-PCR, quantitative reverse transcription PCR. b Genomic sequencing statistics (%) were calculated using KY618133 for MAYV (11,534 nt long) and KP164568 for CHIKV (11,812 nt long) as reference genome.