Skip to main content
. 2020 Jun 26;18(6):335. doi: 10.3390/md18060335

Figure 4.

Figure 4

Protective effects of ASX on NMDA-induced mitochondrial superoxide generation in SH-SY5Y cells. (A) Mitochondrial superoxide levels were detected as a function of time with the MitoSOX probe following addition of 16 µM or 200 µM NMDA. Data were normalized against the fluorescence values obtained before NMDA addition (∆F/F0). *: p < 0.05 compared to addition of 16 µM NMDA. (B) Mitochondrial superoxide levels were measured one minute after addition of 200 µM NMDA to control cells or to cells preincubated for 60 min with 200 µM APV. #: p < 0.05 compared to NMDA. (C) Cells pretreated with increasing concentrations of ASX (from 0.5 nM to 10 µM) for 24 h were incubated with 200 µM NMDA. #: p < 0.05 compared to 200 µM NMDA in the absence of ASX.