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. 2020 Jun 2;9(6):115. doi: 10.3390/biology9060115

Figure 3.

Figure 3

GSI increases protein synthesis and mTOR signaling in C2C12 myotubes. (A) Puromycin/β-Actin; (B) Phospho (p)-mTOR Ser2448/Total mTOR; (C) p-mTOR Ser2481/Total mTOR; (D) p-4EBP1 Thr37/46/Total 4EBP1; (E) p-p70S6K Thr389/Total p70S6K; (F) p-eEF2 Thr56/Total eEF2 expression (Integrated optical density, IOD) in 96-h myotubes treated with or without 4 µm γ-secretase inhibitor (GSI) every 12 h. Thirty minutes prior to collection all cells were treated with 1 µm puromycin. For representative images: lanes 1 and 2 are Con; lanes 3 and 4 are GSI. Data were analyzed using a Student’s T-test. * p ≤ 0.05 vs. Control (Con); ** p < 0.01 vs. Con; **** p < 0.0001 vs. Con (n = 3 experiments). Data are mean ± SD.