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. 2020 Jun 2;9(6):115. doi: 10.3390/biology9060115

Figure 4.

Figure 4

GSI increases signaling upstream of mTOR in C2C12 myotubes. (A) PTEN/β-Actin; (B) p-AKT Thr308/Total AKT; (C) p-AKT Ser473/Total AKT; (D) p-TSC2 Ser939/Total TSC2; (E) p-TSC2 Thr1462/Total TSC2 expression (Integrated optical density, IOD) in 96-h myoblasts treated with or without 4 µm γ-secretase inhibitor (GSI) every 12 h. Thirty minutes prior to collection all cells were treated with 1 µm puromycin. For representative image: lanes 1 and 2 are Con; lanes 3 and 4 are GSI. Data were analyzed using a Student’s T-test. ** p < 0.01 vs. Control (Con); *** p < 0.001 vs. Con; **** p < 0.0001 (n = 3 experiments). Data are mean ± SD.