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(A) Colorimetric LAMP assay of S. Thyphi bacterial DNA in our hard–soft hybrid microfluidic chip (i) before and (ii) after LAMP amplification. (B) Real-time fluorescence monitoring of LAMP amplification of S.Thyphi bacterial DNA with 0, 100, 500, 5,000 CFU per reaction. (C) Threshold time Tt (in minutes), as a function of function of log S.Thyphi bacterial DNA concentration (expressed in terms of CFU per reaction) (n = 3).
