Figure 7.

W Suppression of LRRC8A or LRRC8D subunits in A24 wt cells by siRNA. A24 wt cells were cultivated for two days in absence or presence of siRNA, respectively, and subsequently seeded at densities of 2 × 10⁴ mL⁻¹ and grown in cisPt containing medium (0–3.6 µM). Cell densities were measured after three days. wt (-○-), LRRC8A-siRNA (-○-), LRRC8D-siRNA (-○-), scrambled siRNA (-○-) (A). Knock down efficiency of the LRRC8 protein was checked by SDS-PAGE and western blotting (B). mRNA levels of LRRC8A (C) and LRRC8D (D) was quantified by RT-qPCR. Western blots are representative of three independent repeated experiments. Data are presented as mean ± SD. n equal 3 for mRNA quantification and > 10 for IC₅₀.