Figure 8.

Overexpression of LRRC8A and LRRC8D in (D-)A24cisPt8.0 cells. (D-)A24cisPt8.0 cells were transfected with LRRC8A and / or LRRC8D cDNAs using Lipofectamine™ 2000. As a control, the cells were incubated with Lipofectamine only. Expression was tested by either Western Blot analysis (A) or RT-qPCR (B). Subsequent to expression, the IC₅₀ values for cisPt (C) were determined as described in materials and methods. Western blots are representative of three independent repeated experiments. Data are presented as mean ± SD. n equal 3 for mRNA quantification and >10 for IC₅₀.