Table 1.
Cell pharmacological parameters of the metastatic wt A24 lung adenocarcinoma cell strain and of its sublines with induced and with de-induced resistance to cisPt.
| Starting Subline | A24 wt | A24 wt | A24cisPt0.5 | A24cisPt1.0 | A24cisPt2.0 | A24cisPt4.0 |
|---|---|---|---|---|---|---|
| cisPt concentration exposed to [µM] | n. a. | 0.5 | 1.0 | 2.0 | 4.0 | 8.0 |
| Branching-off after (months) | n. a. | 2 | 2 | 3 | 4 | 12 |
| Resulting subline | n. a. | A24cisPt0.5 | A24cisPt1.0 | A24cisPt2.0 | A24cisPt4.0 | A24cisPt8.0 |
| IC50 cisPt at branching-off time | 0.46 ± 0.05 | 2.13 ± 0.62 | 2.97 ± 0.81 | 6.03 ± 1.39 | 9.09 ± 0.56 | 18.49 ± 1.96 |
| De-induction period (months) | n. a. | 3 | 3 | 7 | 7 | >7 |
| De-induced subline | n. a. | (D-)A24cisPt0.5 | (D-)A24cisPt1.0 | (D-)A24cisPt2.0 | (D-)A24cisPt4.0 | (D-)A24cisPt8.0 |
| IC50 cisPt [µM] | n. a. | 0.89 ± 0.22 | 2.53 ± 0.84 | 0.99 ± 0.07 | 4.02 ± 0.23 | 12.74 ± 0.78 |
| Population doubling time (h) | 20.5 | n. d. | n. d. | 25 | 31 | 42 |
n. a.: not applicable; n. d.: not done; data are presented as mean ± SD. n equal 6 for doubling time and >10 for IC50.