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. 2020 Jun 12;9(6):125. doi: 10.3390/biology9060125

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© 2020 by the authors.

Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).

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Identification and determination of the levels of PIs in the bovine retina. The bovine retina was subjected to sucrose density gradient centrifugation and three fractions were collected (A). Fractions collected from the sucrose gradients were subjected to SDS-PAGE (B). The arrow shows the presence of rhodopsin (40 kDa) in fraction 1. Each panel represents the levels of PI(3)P (C), PI(4)P (D), PI(3,4)P₂ (E), PI(4,5)P₂ (F), and PI(3,4,5)P₃ (G). Data are mean ± SEM (n = 3). The significance is indicated on each panel.