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In situ hybridisation. Ventral views of representative R32 adult brains probed with α-LACZ antibody ((A,D), green), antisense norpA probe (concentrated 75 ng, (B), red)—the bright red staining around the PDF cells may represent trachea, and sense norpA probe (concentrated 150 ng, (E), red). (C) and (F) are a merge of (A,B) and (D,E), respectively. The brains presented are images of 10 independent layers merged together.
