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. 2020 Jun 2;9(6):296. doi: 10.3390/antibiotics9060296

Table 1.

The evolutionary rescue of CRISPR-plasmid harboring antibiotic susceptible E. coli.

Beta-Lactam Susceptible Strain Replicate Survivors (cfu/mL)
1 <200
E. coli DH5α (pCas9-gRNA) 2 <200
3 <200
1 1.52 × 106
E. coli DH5α (pCas9-CTRL) 2 1.76 × 106
3 2.12 × 106