Fig. 1.

Representative images of different LAIR-1 immunohistochemistry staining intensities in OS tissues. The proportion of positively stained cells for LAIR-1 was calculated by assessing the entire image. Based on the LAIR-1 staining intensities in OS tumor samples, the staining patterns were categorized as follows: weak (+), moderate (++), and intense (+++). Upper panel, original magnification × 200; lower panel, original magnification × 400. b Kaplan–Meier plot of survival rates of patients with tumors exhibiting high (blue line) or low (red line) LAIR-1 expression; data were obtained using the R2 platform. c Western blotting for determining LAIR-1 expression and PCNA proliferation marker levels in HOS cells following LV-NC or LV-LAIR-1 lentivirus infection or without treatment (blank). β-actin was used as a loading control