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. 2020 Jun 11;17:26. doi: 10.1186/s12989-020-00357-z

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© The Author(s) 2020

Open AccessThis article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated in a credit line to the data.

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Fig. 1 — Analysis of TiO₂ particle sizes. Size measurement of TiO₂ particles detected in the intestinal lumen of jejunum or colon (lumen Jej and Col), in the jejunal villi and PP (Tissue Villi and PP), in the blood (B) and in the initial suspension (Suspension) before sonication (non-sonicated or NS) or after sonication (S). Confocal size measurement of the TiO₂ particles was performed at high magnification with dual determination by confocal laser reflexion and by classic bright field observation. In addition, the latter S suspension was analysed by TEM (S MET), for comparison. Statistical significant difference between (a) Lumen Jej and Lumen col. (p < 0.001) and (b) in PP compared to B (p < 0.05), S and Lumen Jej (p < 0.001)