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. 2020 Jun 11;17:26. doi: 10.1186/s12989-020-00357-z

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© The Author(s) 2020

Open AccessThis article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated in a credit line to the data.

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Fig. 3 — Confocal and TEM localization of TiO₂ particles in jejunal PP, 4 h after E171 oral administration. a-c Confocal imaging of TiO₂ particles (green) (a) in the SED, (b) in a lymphoid follicle (FAE) or (c) in the FAE monolayer. The tissue auto-fluorescence in shown in red and the DAPI-labelled nucleus in blue. d, e TEM associated to EDX analysis. TiO₂ particles are detected as endocytosed by two separate immune cells of the PP. Bars = 10 μm in confocal microscopy, and 1 μm or 200 nm in TEM