Figure 3.

Olorofim causes cell wall remodeling as indicated by Aniline Blue (β-1,3-glucan) and CFW (chitin) staining. Fluorescence images and measurements are shown on the left and right panels, respectively; error bars represent the SD: (A) Aniline Blue (β-1,3-glucan) staining. No difference in overall fluorescence intensity of mycelial hyphae before and after treatment with 0.1 µg/mL olorofim for 24 h. Scale bar = 40 µm; (B) Aniline Blue (β-1,3-glucan) staining. Reduction in β-1,3-glucan in hyphal tips at the colony periphery following treatment. Scale bar = 10 µm; (C) CFW (chitin) staining. Chitin content was increased in the treated hyphae throughout the mycelium. Scale bar = 40 µm. * p < 0.01.