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Vacuolar volume increases in 24 h olorofim-treated hyphae. Vacuoles in a cytoplasmic-GFP strain were stained with CMAC and the vacuole/cytoplasm ratio was measured. Vacuolar volume was significantly enlarged after exposure to olorofim, resulting in a reduced cytoplasmic volume. Scale bar = 10 µm. Error bars represent SD. * p < 0.01.
