Figure 7.

Olorofim treatment inhibits mitosis. (A) Imaging of H1-GFP nuclei within 100 µm of the hyphal tip at 0 h (Untreated) and 24 h (Treated) following addition of olorofim. Scale bar = 10 µm. Error bars represent SD. (B) After 24 h of olorofim treatment, only 1–2 rounds of mitosis are estimated to take place, based on the accumulated number of nuclei in the hyphal tip. In untreated hyphae, newly formed nuclei move along with the growing hyphal tip, resulting in more equally dispersed nuclei. * p < 0.01.