Table 1.

Live-cell probes, their selectivities, concentrations used, commercial sources and confocal microscope imaging parameters used.

Probes	Selectivity	Concentration	Commercial Source	Excitation Wavelength	Emission Wavelength	Excitation Source
Mitotracker Red FM	Mitochondria	500 ng/mL	Molecular Probes	581 nm	595–650 nm	White light laser
Aniline Blue	Cell wall	25 µg/mL	Sigma-Aldrich	405 nm	420–470 nm	Blue diode laser
Calcofluor White (CFW)	Cell wall chitin	0.1 µg/mL	Sigma-Aldrich	405 nm	420–470 nm	Blue diode laser
Wheat germ agglutinin-orange (WGA-orange)	Cell wall chitin	5 µg/mL	Molecular Probes	555 nm	570–630 nm	White light laser
Cell Tracker Blue/CMAC	Vacuoles	1 µM	Molecular Probes	405 nm	420–470 nm	Blue diode laser
Filipin	Cell membrane ergosterol	1 µg/mL	Sigma-Aldrich	405 nm	420–470 nm	Blue diode laser
Nile Red	Membrane lipids, neutral lipid droplets	10 µg/mL	Sigma-Aldrich	514 nm	530–600 nm	White light laser
FM4-64	Membrane	5 µM	Molecular Probes	515 nm	550–700 nm	White light laser
GFP	Various	Not applicable	This study	488 nm	500–550 nm	White light laser