Table 1.
Results obtained for each response variable at the different extraction condition of Euglena cantabrica defined by the design of experiments of pressurized liquid extraction (PLE): yield, TPC, and antioxidant capacities obtained by ABTS and DPPH assays. GAE: gallic acid equivalents per gram of extract, EtOH: solvent composition, TE: Trolox equivalents, and EC50: effective concentration of extract (in µg/mL) able to decrease the DPPH radical concentration by 50%.
| Experiment # | T, °C | %EtOH (v/v) | Extraction Yield, %(m/m) | TPC, mg GAE/g Extract |
ABTS, mmol TE/g Extract |
DPPH, EC50 (µg/mL) |
|---|---|---|---|---|---|---|
| Center (a) | 110 | 50 | 21.6 ± 1.1 | 45.6 ± 6.9 | 0.80 ± 0.02 | 26.8 ± 3.9 |
| 2 | 180 | 50 | 39.1 | 38.3 | 1.26 | 27.6 |
| 3 | 110 | 0 | 9.9 | 16.8 | 0.46 | 66.2 |
| 4 | 40 | 0 | 15.9 | 14.5 | 0.39 | 132.2 |
| 7 | 180 | 100 | 12.1 | 39.4 | 0.55 | 7.5 |
| 9 | 180 | 0 | 27.3 | 46.0 | 1.28 | 29.7 |
| 10 | 110 | 100 | 11.6 | 30.1 | 0.32 | 33.2 |
| 11 | 40 | 100 | 3.2 | 19.5 | 0.22 | 67.8 |
| 12 | 40 | 50 | 12.9 | 34.3 | 0.73 | 30.0 |
(a)Average results (± SD) from the four replicates of the center points of the design of experiments (DoE) corresponding to the experiments 1, 5, 6, and 8.