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. 2020 May 28;11(6):546. doi: 10.3390/mi11060546

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© 2020 by the authors.

Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).

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Statistical comparison of the normalized neurite length and percentages of neurons with outgrowths. (a) Uniform samples. The median normalized neurite length after 3 days in vitro (DIV) is greatest for neurons grown on the Al/Fe preparation. Statistical analysis revealed that the Al/Fe preparation was significantly greater than the Al/Fe+Pl and Fe+Pl preparations. (b) Uniform samples. The percentage of neurons with neurite outgrowths after 3 DIV is greatest for neurons grown on the Al/Fe preparation. Statistical analysis revealed that the Al/Fe preparation was significantly greater than the Al/Fe+Pl and Fe+Pl preparations. (c) Patterned samples. The median normalized neurite length was seen to have a statistically significant increase between 3 and 7 DIV, demonstrating the biocompatibility of the Al/Fe group at longer culture times. Each block dot represents the average value for one of the samples. ****, ***, and ** correspond to p values <0.0001, <0.005, and <0.01 respectively.