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Validity of the method in terms of standard curve linearity and precision.
| Vitamin | Coefficient of Determination (R2) | Intraday CV | Interday CV |
|---|---|---|---|
| B2 (Riboflavin) | 0.9971 | 6.6 | 61.5 |
| B3 (Nicotinamide) | 0.9977 | 3.7 | 6.7 |
| B5 (Pantothenic acid) | 0.9968 | 3.0 | 20.7 |
| B6 (Pyridoxine) | 0.9997 | 1.0 | 3.6 |
| B7 (Biotin) | 0.9963 | 3.0 | 4.9 |
Precision was determined by the intraday and interday coefficients of variation (CV), determined by the standard deviation /mean.
