Table 3.
Extraction efficiency and percentage recovery estimation for new method.
| Vitamin | Raw Sample (ng/µL) | Spiked Sample (Pre-Extraction) (ng/µL) |
Spiked Sample (Post-Extraction) (ng/µL) |
Pure Vitamin Cocktail (ng/µL) |
Extraction Efficiency (%) |
Matrix Effects (%) |
Recovery (%) |
|---|---|---|---|---|---|---|---|
| B2 (Riboflavin) | 0.0199 | 2.841 | 5.788 | 3.724 | 49.1 | 155.4 | 75.8 |
| B3 (Nicotinamide) | 0.0388 | 0.971 | 1.650 | 2.328 | 58.8 | 70.1 | 40.0 |
| B5 (Pantothenic acid) | 0.2297 | 3.301 | 5.99 | 3.781 | 55.0 | 158.4 | 81.2 |
| B6 (Pyridoxine) | 0.0187 | 2.777 | 5.306 | 4.029 | 52.3 | 131.6 | 68.5 |
| B7 (Biotin) | 0.0549 | 2.608 | 5.600 | 4.086 | 46.6 | 137.0 | 62.5 |
Descriptions of the raw sample as well as the spiked pre- and post-extraction samples are described in Materials and Methods. Extraction efficiency was calculated by the concentration of the pre-extraction spiked sample as a percentage of the concentration of the post-extraction spiked sample. Matrix effects were calculated by dividing the vitamin concentration of the post-extraction spiked sample by the concentration of each vitamin obtained from the pure standard solution. Recovery was calculated using the difference between the B vitamin concentration in the spiked and the raw unspiked sample, expressed as a percentage of the concentration of each vitamin obtained from the pure standard solution.