Table 5.
Summary of B vitamin analysis of HPLC–MS/MS method.
| Vitamin Analyte | High Linearity | High Inter and Intraday Precision | Low Limit of Detection | Matrix Effects | High Recovery |
|---|---|---|---|---|---|
| B1 (Thiamine) | − | N/A | N/A | N/A | N/A |
| B2 (Riboflavin) | + | +/− | + | enhanced | + |
| B3 (Nicotinamide) | + | + | + | suppressed | − |
| B5 (Pantothenic acid) | + | +/− | + | enhanced | + |
| B6 (Pyridoxine) | + | + | + | enhanced | − |
| B7 (Biotin) | + | + | − | enhanced | − |
| B9 (Folic acid) | − | N/A | N/A | N/A | N/A |
We evaluated acceptability in terms of linearity, if the value was >99.5%; precision, if it was <15%; recovery, if it was >75%; limit of detection, if it was 5-fold or more lower than the reference range; and matrix effects as high, if it was >100%; and low, if it was <100%.