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. Author manuscript; available in PMC: 2020 Jul 9.
Published in final edited form as: Neurobiol Dis. 2019 Oct 13;134:104626. doi: 10.1016/j.nbd.2019.104626

Fig. 5.

Fig. 5.

Rotenone causes accumulation of early endosomes prior to deficits in macroautophagy in rats. The number of early endosomes in dopaminergic neurons were assessed by immunohistochemistry using the early endosomal specific marker Rab5 and the dopaminergic specific marker TH in aged rats. Representative photomicrographs of Rab5 (Red) in nigral TH+ (Green) neurons in rats dosed with vehicle, acute or endpoint rotenone (A). The quantification for the average number of Rab5 puncta per dopaminergic neuron; n = 4/grp (B). The number of autophagosomes in dopaminergic neurons were assessed by immunohistochemistry using the p62/SQSTM1 and TH (C). The quantification for the average number of p62/SQSTM1 puncta per dopaminergic neuron; n = 4/grp (D). Data are analyzed using unpaired t-test * p < .05, ** p < .001, *** p < .001 graphs are expressed as mean ± SEM. Symbols represent individual brains. (For interpretation of the references to colour in this figure legend, the reader is referred to the web version of this article.)