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. 2019 Aug 14;28(20):3431–3442. doi: 10.1093/hmg/ddz181

Figure 4.

Figure 4

Mmp9 contributes to proteolytic processing of TGFβ precursor. (A) Schematic structure of SLC and LLC of TGFβ precursor that can both be cleaved by Mmp9. (B) CM of fibroblasts incubated with or without Mmp9 inhibitor shown in triplicate (Enzo Life Sciences) were probed with anti-TGFβ antibody. Arrow indicates 80 kDa SLC, which is accumulated in CM containing Mmp9 inhibitor. (C) Inhibition of Mmp9 activity was associated with a dose-dependent decrease in collagen I expression. (D) Schematic of a genetic cross to produce Mmp9−/−mdxB10 double mutant mice. (E) Western blot analysis of total protein lysates from Mmp9−/−mdxB10 and Mmp9+/+mdxB10 mice (n = 4 of each genotype) showed decreased active form of TGFβ (25 kDa) in Mmp9-deficient mice. Quantitative analysis of the western blot is shown in (F). Asterisk indicates statistical significance of P < 0.05; AU, arbitrary units.