Skip to main content
. 2020 May 29;10(6):225. doi: 10.3390/metabo10060225

Figure 2.

Figure 2

MS characteristics of Cer (d18:1/16:0). (A) Relative intensities of [M−H]−, [M+OAc]−, and [M+HCOO]− ions. (B–D) Fragmentation patterns of the [M−H]− ion formed from Cer (d18:1/16:0) under negative ionization conditions: relative intensities versus collision energy of (B) precursor ion, (C) fatty acyl side chains, and (D) sphinganine ions. (E) Tandem mass spectrum of Cer (d18:1/16:0) [M−H]− ion obtained by collision energy ramping from 20 to 40 eV. Product ions: T (m/z 280.2646), U (m/z 254.2486), and S (m/z 296.259) are indicative of the fatty acyl chains; Q (m/z 263.2379) and P (m/z 237.2225) are indicative of the sphingosine moiety. Product ions are labelled according to Reference [37]. Green and red colors relate to sphingosine and fatty acyl position, respectively.