Figure 1.

Effect of maize root powder on M. maydis extracellular laccase activity over time: The fungus was grown for seven days in 50 mL of a minimal medium (MM) with 0.1% glucose to which 750 mg of shredded plant parts in liquid nitrogen from 40-day-old Prelude cv. maize plant was added. The control is MM with M. maydis, without root powder. To the growth medium of each treatment and the control (after purification), 2,2′-azinobis (3-ethylbenzothiazoline-6-sulfonic acid) (ABTS) was added. The enzymatic units (U) were defined as the amount of enzyme oxidizing 1 µmol ABTS in 1 min at 28 ± 1 °C in the dark. The values shown are an average of five repetitions.