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. 2020 May 17;6(2):63. doi: 10.3390/jof6020063

Table 1.

Primers for Magnaporthiopsis maydis qPCR detection. 1

Pairs Primer Sequence Uses Amplification References
Pair 1 A200a-for
A200a-rev
5′-CCGACGCCTAAAATACAGGA-3′
5′-GGGCTTTTTAGGGCCTTTTT-3′
Target gene M maydis AFLP-derived species-specific fragment [24]
Pair 2 COX-F
COX-R
5′-GTATGCCACGTCGCATTCCAGA-3′
5′-CAACTACGGATATATAAGRRCCRRAACTG-3′
Control Cytochrome c oxidase (COX) gene product [38,39]

1 qPCR—quantitative real-time PCR. AFLP—amplified fragment length polymorphism.