Table 1.
Pairs | Primer | Sequence | Uses | Amplification | References |
---|---|---|---|---|---|
Pair 1 | A200a-for A200a-rev |
5′-CCGACGCCTAAAATACAGGA-3′ 5′-GGGCTTTTTAGGGCCTTTTT-3′ |
Target gene | M maydis AFLP-derived species-specific fragment | [24] |
Pair 2 | COX-F COX-R |
5′-GTATGCCACGTCGCATTCCAGA-3′ 5′-CAACTACGGATATATAAGRRCCRRAACTG-3′ |
Control | Cytochrome c oxidase (COX) gene product | [38,39] |
1 qPCR—quantitative real-time PCR. AFLP—amplified fragment length polymorphism.