. 2020 Jun 26;9(6):138. doi: 10.3390/biology9060138

Table 3.

Kinematic variables (means ± SEM) of motile bull sperm SPs in different commercial extenders.

Kinematic Variables	VCL	VSL	VAP	LIN	STR	WOB	ALH	BCF
OptiXcell^®
SP₁	120.62 ± 1.5^aβ	74.41 ± 1.35^aβ	77.17 ± 1.32^aβ	59.36 ± 0.67^aβ	91.87 ± 0.53^aβ	62.48 ± 0.57^aβ	3.96 ± 0.05^aβ	11.38 ± 0.13^aβ
SP₂	165.50 ± 1.20^bβ	144.29 ± 1.07^bβ	143.07 ± 1.04^bβ	87.16 ± 0.53^bβ	97.52 ± 0.42^bβ	86.77 ± 0.45^bβ	3.80 ± 0.04^aβ	10.00 ± 0.10^bβ
SP₃	128.03 ± 1.95^cβ	43.81 ± 1.74^cβ	81.97 ± 1.70^aβ	31.53 ± 0.86^cβ	50.71 ± 0.68^cβ	62.23 ± 0.73^cβ	4.49 ± 0.06^bβ	6.73 ± 0.17^cβ
SP₄	86.70 ± 1.36^dβ	74.24 ± 1.22^aβ	77.73 ± 1.19^aβ	83.07 ± 0.60^dβ	92.64 ± 0.48^aβ	88.08 ± 0.51^dβ	2.05 ± 0.05^cβ	6.76 ± 0.12^cβ
Triladyl^®
SP₁	129.45 ± 1.20^aγ	111.73 ± 1.19^aγ	111.19 ± 1.02^aγ	85.91 ± 0.61^aγ	97.22 ± 0.66^aγ	86.06 ± 0.47^aγ	3.29 ± 0.04^aγ	8.62 ± 0.11^aγ
SP₂	74.94 ± 1.57^bγ	31.09 ± 1.57^bγ	42.28 ± 1.35^bγ	41.45 ± 0.80^bγ	72.34 ± 0.87^bγ	55.77 ± 0.62^bγ	2.97 ± 0.06^bγ	7.56 ± 0.15^bγ
SP₃	168.80 ± 1.27^cγ	79.89 ± 1.56^cγ	96.62 ± 1.34^cγ	46.80 ± 0.80^cγ	80.53 ± 0.87^cγ	57.38 ± 0.62^bγ	6.17 ± 0.06^cγ	8.80 ± 0.15^aγ
SP₄	158.77 ± 1.10^dγ	120.00 ± 1.09^dγ	119.45 ± 0.94^dγ	75.78 ± 0.56^dγ	97.66 ± 0.61^aγ	75.52 ± 0.43^cγ	4.31 ± 0.04^dγ	14.13 ± 0.10^cγ
Tris-EY
SP₁	108.68 ± 1.64^aδ	30.28 ± 1.65^aδ	66.08 ± 1.54^aδ	25.85 ± 0.88^aδ	43.95 ± 0.62^aδ	59.21 ± 0.78^aδ	3.94 ± 0.06^aβ	6.54 ± 0.17^aδ
SP₂	137.08 ± 1.07^bδ	122.67 ± 1.07^bδ	124.09 ± 1.00^bδ	88.45 ± 0.57^bβ	95.11 ± 0.40^bδ	90.79 ± 0.51^bδ	2.85 ± 0.04^bγ	8.39 ± 0.11^bδ
SP₃	74.15 ± 1.14^cδ	45.17 ± 1.15^cβ	48.47 ± 1.07^cδ	58.92 ± 0.61^cδ	88.19 ± 0.43^cδ	64.62 ± 0.54^cδ	2.66 ± 0.04^cδ	8.49 ± 0.12^bγ
SP₄	168.04 ± 0.97^dδ	118.06 ± 0.97^dγ	118.09 ± 0.90^dγ	70.01 ± 0.52^dδ	96.56 ± 0.37^dγ	70.22 ± 0.46^dδ	4.96 ± 0.03^dδ	12.62 ± 0.10^cδ

VCL = curvilinear velocity (µm·s⁻¹); VSL = straight-line velocity (µm·s⁻¹); VAP = average path velocity (µm·s⁻¹); LIN = linearity of forward progression (%); STR = straightness (%); WOB = wobble (%); ALH = amplitude of lateral head displacement (µm); BCF = beat-cross frequency (Hz); SP: subpopulation; SEM = standard error of the mean. Number of cells = 6493. ^a-d Within the same column and extender, different superscripts indicate differences among sperm SPs. ^βγδ Within the same column and SP, different superscripts indicate differences among extenders. p < 0.05.