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. 2020 Jun 9;12(11):11025–11041. doi: 10.18632/aging.103314

Figure 2.

Figure 2

LncRNA ADAMTS9-AS2 acted as a RNA sponge to regulate miR-223-3p in GC cells. (A) The targeting sites of LncRNA ADAMTS9-AS2 and miR-223-3p were predicted by using the online starBase software (http://hopper.si.edu/wiki/mmti/Starbase). Dual-luciferase reporter gene system was used to verify the binding sites in (B) SGC7901 cells and (C) BGC-823 cells, respectively. (D) RIP was performed to measure the binding abilities of LncRNA ADAMTS9-AS2 and miR-223-3p. Real-Time qPCR was used to examine the expression levels of (E) LncRNA ADAMTS9-AS2 and (F) miR-223-3p in GC cells. Each experiment repeated at least 3 times. “NS” represented “no statistical significance”, *P < 0.05, **P < 0.01.