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. 2020 May 21;12(11):10517–10526. doi: 10.18632/aging.103276

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Copyright © 2020 Zheng et al.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY 3.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.

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SOCS2-AS1 overexpression inhibited CRC proliferation, migration and invasion. (A) SOCS2-AS1 expression was upregulated in HCT116 and SW480 cells after transfection with SOCS2-AS1 overexpressing vector. (B, C) CCK8 and colony formation assays were performed to determine CRC cell proliferation. (D) Cell proliferation was evaluated by immunofluorescence staining with EdU. (E) Cell-cycle status of CRC cells were analyzed by FACS. (F, G) Transwell assay was performed to analyze cell migration and invasion. *P<0.05.