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. 2020 May 20;12(11):10457–10472. doi: 10.18632/aging.103270

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Copyright © 2020 Wang et al.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY 3.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.

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The protective effects of EA on glutamate-induced toxicity in neuronal cells. (A) The expression levels of Nrf2 and TLR4 significantly decreased in the siRNA treatment group. (B) and (C) Effect of EA on ROS levels in Nrf2 or TLR4 siRNA-transfected and Glu-treated neuronal cells. (D) and (E) Effect of EA on cell viability in Nrf2 or TLR4 siRNA-transfected and Glu-treated neuronal cells. Data values were expressed as the mean ± SEM (n=3), ^##P < 0.01 vs. control group; ^*P < 0.05 and ^**P < 0.01 vs. Glu group; ^&P < 0.05 and ^&&P < 0.01 vs. EA-treated Glu group.