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Treatment of N-acetyl-l-cysteine (NAC) in vitro attenuates ROS levels, ERS levels in NEFA treated GCs. (A) The accumulation of ROS was observed by immunofluorescence (scale bar, 200 μm). The results showed that NAC inhibited the accumulation of ROS induced by NEFA. (B–E) Western blotting showing protein expression levels of CHOP, GRP78, and p-PERK, which indicate that NAC inhibited ERS. Data are presented as mean ± SEM, * p < 0.05, and ** p < 0.01.
