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Treatment of NAC in vitro attenuates apoptosis in NEFA treated GCs. (A,E) Apoptosis events were determined by FACS analysis after Annexin V-Alexa Fluor 647/PI staining in the treated GCs. (B–D) The results of Bax, Bcl-2, and cleaved caspase-3 protein levels showed that NAC alleviated GCs apoptosis induced by high-level NEFA. Data are presented as mean ± SEM, * p < 0.05, ** p < 0.01, and *** p < 0.001. ns, not significant.
