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The ROS mediated apoptosis induced by NEFA is regulated by Nrf2/HO-1 and p53/Bax signaling pathways. (A) The nuclear levels of Nrf2 and p53 were detected by immunoblotting. (B,C) The interaction between p53 and Bax is detected by performing co-immunoprecipitation (co-IP) experiments in GCs. The results showed that Nrf2/HO-1 and p53/Bax signaling pathways were involved in NEFA induced apoptosis. “+”, add; “-” without add.
