Figure 5.

Effects of OGDR, phytoestrogens, and 3-MA treatment on AKT and MAPK phosphorylation. Western blots of ⁴⁷³SerP-AKT (A,B,D) and ²⁰²P-Thr- and ²⁰⁴P-Tyr-P-MAPK 42,44 (A–C) were performed in extracts of cortical neurons subjected to 3 h OGD exposure (O) and 24 h OGDR (OR), in the absence or presence of 5 mM 3-MA (A,B), or in the presence of 1 μM Gen or BE with or without 5 μM PD98059 or 5 μM LY294002 (C,D). Upper panels in (C,D) show representative Western blots. Lower panels represent the quantitative analysis of indicated ratios and are mean ± SEM of three experiments, each one performed in duplicate. Statistics in panel B compare the effect of OR over their respective controls (***) or the effect of 3-MA in each condition versus respective conditions in the absence of this compound (ooo). Statistics in panels (C,D) compare the effect of OR alone versus C24h and after addition of phytoestrogens (***) or the effect of PD or LY in each condition versus the same conditions in the absence of these compounds (^ooo) at ^o p < 0.05, ** p < 0.01, *** p < 0.001 (one-way ANOVA test).