Figure 4.

Blocking catalase attenuates the activity of PRF lysate in preventing cell death. Gingival fibroblasts (A) were exposed to 10% PRF in the presence or absence of 3 mM H₂O₂ and 100 mM catalase inhibitor, aminotriazole. Cell viability is measured by the conversion of MTT into formazan crystals. (B) MTT assay also demonstrated that heated PRF is not able to neutralize H₂O₂. n = 3–5. Data represent the mean ± SD. * p < 0.05, ** p < 0.01, by two-tailed Mann–Whitney test.