Figure 5.

PRF capacity in neutralizing hydrogen peroxide is dose-dependent. (A) Gingival fibroblast cells were exposed to PRF lysates in the presence of various doses of H₂O₂ (10, 30, 100, 300 mM). MTT results confirmed cell viability in the presence of PRF lysates for all the groups except 300 mM. (B) The neutralizing capacity of PRF lysates incubated at 37 °C for 24, 48, and 72 h were also tested in the presence of 3 mM H₂O₂. The results suggest that catalase activity was decreased in a time-dependent manner. n = 3–5. Data represent the mean ± SD. *** p < 0.001 and ns = not significant, by two-tailed Mann–Whitney test.