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. 2020 Jun 1;9(6):470. doi: 10.3390/antiox9060470

Figure 5.

Figure 5

GOFA effects on apoptosis and cell senescence. The effect of GOFA on apoptosis of U937 (a) and HCT116 (b) cells. The cells were treated with indicated concentration of GOFA for 1 h and then exposed to LPS for 24 h. An Annexin V assay was used for apoptosis detection. (c) Bar diagram showing specific effect of GOFA on caspase 3 fluorescent activity on LPS-treated U937 and (d) HCT116 cells; (e) evaluation of cellular senescence effects of GOFA on U937 and (f) HCT116 cells. Cellular senescence was measured using the cellular senescence assay, in which the SA-β-Gal activity was normalized to total protein concentration. All values represent the mean ± SD of three independent experiments. * p < 0.05 vs. control cells; # p < 0.05 vs. cell treated with LPS alone.