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. 2020 Jun 1;9(6):470. doi: 10.3390/antiox9060470

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© 2020 by the authors.

Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).

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GOFA effects on MMP-9 for U937 (a) and HCT-116 (b) cell lines. Cell-free conditioned media were assayed for MMP-2 and MMP-9 activity by gelatin zymography. The activity of MMP-9 (92 kDa) (bottom) was reported with fold induction values compared to control MMP-9 activity. Zymogram (top) is representative of 6 gels using 3 separate pools of total protein extracted from cell lines. Changes in MMP-9 gene expression were determined by qRT-PCR assay, using the (ΔΔCt) method and 18S as housekeeping gene. One-way ANOVA, values represent mean ± SD (n = 6); ^* p < 0.05 vs. control cells; ^# p < 0.05 vs. cell treated with LPS alone.