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Biotin Switch assay of s-nitrosylated proteins. (a) Blots and corresponding Ponceau staining of s-nitrosylated proteins in homogenates of goldfish hearts perfused under either normoxia (N) or hypoxia (H). (b) Densitometric analysis of normalized S-NO (biotin)/Ponceau signal. Data were expressed as means ± s.e.m. of absolute values from individual experiments (n = 3); statistics were assessed by two-tailed unpaired t-test (* p < 0.05).
