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. 2020 Jun 9;9(6):507. doi: 10.3390/antiox9060507

Figure 2.

Figure 2

BAI inhibits BaP-induced CYP1A1 expression in keratinocytes. (a) NHEKs or (b) HaCaT cells were treated with DMSO (0.1%), BAI (10 μM for NHEKs and 25 μM for HaCaT cells), BaP (1 μM) or a combination of BAI and BaP and assessed for CYP1A1 mRNA expression (upper panels) at 6 h- or CYP1A1 protein expression (middle and lower panels) at 24 h-post treatment. (c) HaCaT cells were treated with DMSO (0.1%), BAI (25 μM), BaP (1 μM) or a combination of BaP and BAI, BaP and WO, or BaP and OG, and assessed for CYP1A1 mRNA expression (upper panels) at 6 h or CYP1A1 protein expression (middle and lower panels) at 24 h post treatment. Experiments were performed in triplicate wells and each experiment was repeated three times. Representative blot images (middle panels) and mean ± SD of CYP1A1/β-ACTIN ratio (lower panels) are shown. Whole blot images are shown in Supplementary Figure S2. * p < 0.05, ** p < 0.01 and *** p < 0.001.

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