Figure 8.

BaP-induced AHR nuclear translocation is prevented by BAI. (a) HaCaT cells were treated with DMSO (0.1%), BaP (1 μM), the combination of BaP and dasatinib (100 nM) or the combination of BaP and BAI (25 μM) for 24 h and nuclear translocation of AHR was examined using immunocytochemistry. E-cadherin staining indicates plasma membrane and arrows indicate AHR that translocated to the nucleus. DAPI was used for nuclear staining. Scale bar = 100 μm. (b) HaCaT cells were treated as in (a) and cytoplasmic and nuclear fraction were separately extracted. β-ACTIN and Lamin B1 were used as internal controls of cytoplasmic or nuclear protein. Representative blot images (upper panels) and mean ± SD of relative AHR/β-ACTIN and AHR/Lamin B1 ratio (lower panels) are shown. Experiments were performed in triplicate wells and each experiment was repeated three times. Whole blot images are shown in Supplementary Figure S8c. * p < 0.05 and *** p < 0.001.