Figure 7.

E3330 effect on collective and chemotactic migration of H1975 cells exposed to cisplatin. Collective cell migration was evaluated by the wound-healing assay (A) and chemotaxis was measured using a transwell assay (C). Representative microscopy images of the wound-healing assay (40×, B) and the chemotaxis assay (migrating cells stained with crystal violet—100×, D). Scale bars = 200 μm. Values for the wound-healing assay represent mean ± SD (n = 3) and are expressed as percentage of wound closure, calculated relative to the initial width; * p < 0.05 and ** p < 0.01 (Student’s t-test). Values for the chemotaxis assay represent mean ± SD (n = 3) and are expressed as percentages relative to vehicle-treated control cells; * p < 0.05 (Student’s t-test).