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. 2020 Jun 5;9(6):491. doi: 10.3390/antiox9060491

Figure 4.

Figure 4

IL-6/JAK2/STAT3 cascades participated in CRAMP inhibited VSMC proliferation and migration. (A) VSMC were pretreated with IL-6 antibody (10 μg/mL) for 2 h and CRAMP (100 ng/mL) for 2 h and then stimulated with PDGF-BB (20 ng/mL) for 24 h. The proliferation of VSMC was detected by EdU assay. (B) VSMC were pretreated with IL-6 antibody (10 μg/mL) for 2 h and CRAMP (100 ng/mL) for 2 h and then stimulated with PDGF-BB (20 ng/mL) for 24 h. The migration of VSMC was detected by wound healing assay. (C) VSMC were pretreated with IL-6 (10 ng/mL) for 2 h and CRAMP (100 ng/mL) for 2 h and then stimulated with PDGF-BB (20 ng/mL) for 24 h. The proliferation of VSMC was detected by EdU assay. (D) VSMC were pretreated with recombinant IL-6 at final concentration of 10 ng/mL and CRAMP (100 ng/mL) for 2 h and then stimulated with PDGF-BB (20 ng/mL) for 24 h. Data of 3 independent experiments is presented as mean ± SEM. * p < 0.05, ** p < 0.01, *** p < 0.001 compared with control, n = 4.