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. 2020 Jun 5;9(6):491. doi: 10.3390/antiox9060491

Figure 5.

Figure 5

CRAMP regulated IL-6 autocrine via targeting NF-κB signaling. (A) VSMC were pretreated with CRAMP (100 ng/mL) for 2 h and then stimulated with PDGF-BB (20 ng/mL) at different times (0.5, 1, 2, 4, 6 h). The nuclear- and cyto-p65 were detected by immunoblotting. (B) VSMC were pretreated with CRAMP (100 ng/mL) for 2 h and then stimulated with PDGF-BB (20 ng/mL) at different times (0.5, 1, 2, 4, 6 h). The activation of inhibitor of nuclear factor kappa-B kinase (IKKα/β) was detected by immunoblotting. Data of 3 independent experiments is presented as mean ± SEM. * p < 0.05, ** p < 0.01, *** p < 0.001 compared with control; # p < 0.05, ## p < 0.01, ### p < 0.001 compared with PDGF-BB treatment group, n = 3.