. 2020 May 27;9(6):458. doi: 10.3390/antiox9060458

Table 3.

Michaelis-Menten kinetic parameters on different substrates as action of cocoa bean PPO.

Substrate	K_m [mM]	v_max (U_PPO mL⁻¹ min⁻¹)	v_max/K_m (U_PPO/mM mL min)
Catechol	0.61 ± 0.12 ^a	2709 ± 21.89 ^a	4440.98
Catechol *	8.36 ± 1.33 ^b	106.7 ± 3.70 ^b	12.76
(−)-Epicatechin	1.26 ± 0.37 ^a	916.5 ± 20.59 ^c	727.38
(+)-Catechin	1.45 ± 0.35 ^a	924.8 ± 18.98 ^c	637.79

* Substrate evaluated using PPO enzyme from inactivated cocoa beans at optimum conditions (70 mM inhibitory solution at 96 °C for 6.4 min). Means within a column sharing the same letter are not significantly different by Tukey (p > 0.05).